Actin, Muscle Specific

Actin is a major component of the cytoskeleton. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-Muscle-Specific Actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. This antibody is useful in the identification of rhabdoid cellular elements.\n\nApplications:\nSuitable for use in Immunohistochemistry.Other applications have not been tested.\n\nRecommended Dilutions: \nImmunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. \nOptimal dilution to be determined by the researcher.\n\nPositive Control:\nMuscle\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0760-22

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum, Rat, Rabbit

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1,k

References

1. Tsukada T. et. al. HHF35, a muscle-actin specific monoclonal antibody. I. Immuno-cytochemical and biochemical characterization. Am J Pathol 1987, 126:51. 2. Tsukada T. et. al. HHF35, a muscle-actin specific monoclonal antibody. II. Reactivity in normal, reactive and neoplastic human tissues. Am J Pathol 1987, 127:389.

Additional Info

Recognizes human actin from tissue extracts of uterus, ileum, aorta, diaphragm, heart, and smooth muscle cells. Recognizes alpha actin from skeletal, cardiac, and smooth muscle, and gamma actin from smooth muscle sources. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). \nSpecies Crossreactivity: rabbit and rat.