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Actin, Muscle Specific

Cat no: A0760-22B

Actin, Muscle Specific

Applications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (formalin/paraffin): 1:25-1:50 for 30 min at RT.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMuscle or sarcoma\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0760-22B

Size

250ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum, Rat, Rabbit

Form

Supplied as a liquid with 0.09% sodium azide before the addition of glycerol to 40%.

P Type

Mab

Purity

Tissue culture supernatant.

Isotype

IgG1

References

1. Tsukada T. et. al. HHF35, a muscle-actin specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. Am J Pathol 1987, 126:51. 2. Tsukada T. et. al. HHF35, a muscle-actin specific monoclonal antibody. II. Reactivity in normal, reactive and neoplastic human tissues. Am J Pathol 1987, 127:389.

Additional Info

Recognizes actin from tissue extracts of uterus, ileum, aorta, diaphragm, heart, and smooth muscle cells. It recognizes the alpha actin from skeletal, cardiac, and smooth muscle and the gamma actin from smooth muscle sources. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). Species Crossreactivity: human, rabbit and rat.

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