Actin, pan

Cellular Localization: Cytoplasmic\n\nPositive Control: HeLa cells, Skeletal muscle\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 42kD band is seen.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0760-51C

Size

500ul

Applications

IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, Ch/Bird, Prc, Rabbit

Form

10mM PBS, pH7.4, 0.2%BSA

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

Lessard J. (1988) Two Monoclonal antibodies to actin: One muscle reactive and one generally reactive. Cell Motility and Cytoskeleton 10: 349- 362.

Additional Info

This antibody reacts with all six known vertebrate isoforms of actin (MW 42kDa). It also reacts with two cytoplasmic actins (beta, lambda) which are highly homologous to one another but which differ from the muscle actins at about 25 amino acid residues. This antibody is highly recommended for monitoring total protein load on Western blots. Species Crossreactivity: human, chicken, bovine, canine, mouse, porcine, rabbit, Rat, Dictyostelium discoideum and Physarum polycephalum.