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Actin, Smooth Muscle (alpha-Actin 2, ACTA2)

Actin, Smooth Muscle (alpha-Actin 2, ACTA2)

Cat no: A0760-33B

Supplier: United States Biological
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Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1000 Western Blot: 0.1-0.3ug/ml. ~45kD band observed in human duodenum and heart lysates (calculated MW: 42kD). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer,
Catalogue number: A0760-33B
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-saline, pH7.3, containing 0.5% BSA and 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: ACTSA, ACTVS, ACTSA, alpha Smooth Muscle Actin
References: Reddy S, Ozgur K, Lu M, Chang W, Mohan SR, Kumar CC, Ruley HE. Structure of the human smooth muscle alpha-actin gene. Analysis of a cDNA and 5' upstream region. J Biol Chem. 1990 Jan 25;265(3):1683-7. PMID: 2295650
Additional info: Recognizes human actin alpha 2. Expected to react with mouse, rat and canine based on sequence similarity.

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