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Actinin, alpha 1

Cat no: A0761-09


Supplier: United States Biological
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Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. Alpha-actinin-1 and -4 (ACTN1, ACTN4) are non-muscle isoforms version of ACTN. These isoform are commonly expressed in filopodia, lamellipodia, stress fibers, sites of adhesion and intercellular contacts (2). Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:40 Western Blot: 1:500-1:1000 Immunoprecipitation: 1:40 Immunocytochemistry: 1:100-1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0761-09
Reactivities: Human
Hosts: Rabbit
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
P type: Mab
Isotype: IgG
Purity: Serum
References: 1. Ylanne J, et al. Structure 9(7): 597-604, 2001. 2. Otey, CA and Carpen O. Cell Motil. Cytoskeleton 58: 104-111, (2004).
Additional info: Recognizes human Actinin, alpha 1 at ~100kD.

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