Actinin, alpha 1 (Alpha Actinin 1, alpha-Actinin-1, ACTN1, alpha-Actinin Cytoskeletal Isoform, F-actin Cross-linking Protein, FLJ40884, FLJ54432, Non-muscle alpha-Actinin-1)

a-actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently, a-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration and immune cell targeting. (1). The interaction of a-actinin with ICAM5 (intercellular adhesion molecule-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of a-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). Isoforms 1 and 4 of a-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).\n\nApplications:\nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence (IC): 1:25\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0761-02E

Size

100ul

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1.Otey C.A. et al., Cell Motil Cytoskeleton 58:104-111 (2004) 2. Nyman-Huttenen H et al., J Cell Sci. 119:3057-3066 (2006). 3. Triplett J.W. et al., Am. J. Physiol Cell Physiol 291: C909-921 (2006).

Additional Info

Reognizes endogenous levels of total human alpha-actinin-1 protein. Based on sequence homology, the antibody may cross-react with alpha-actinin isoforms 2 and 4. Species crossreactivity: mouse, rat, hamster and monkey.