Actinin, alpha

Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin. \n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 3.5ug/ml\nImmunohistochemistry (Paraffin, frozen): 3.5-7ug/ml in formalin fixed, paraffin embedded sections and formalin or acetone fixed tissues. Boiling paraffin sections is required.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0762-01

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml sterile PBS.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG1

References

1. Youssoufian, H.; McAfee, M.; Kwiatkowski, D. J. : Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Am. J. Hum. Genet. 47: 62-72, 1990.

Additional Info

Recognizes alpha-Actinin. Species Crossreactivity: human, mouse and rat.