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Actinin, alpha (alpha-Actinin, alpha-Actinin Cytoskeletal Isoform, F-actin Cross-linking Protein, Non-muscle alpha-Actinin)

Actinin, alpha (alpha-Actinin, alpha-Actinin Cytoskeletal Isoform, F-actin Cross-linking Protein, Non-muscle alpha-Actinin)

Cat no: A0762-01B

Supplier: United States Biological
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Alpha-actinin is a cytoskeletal protein located in the Z band/disc region of skeletal muscle where it cross-links with F-actin. The presence of alpha-actinin can be related to a condition known as congenital nemaline myopathy. This antibody does not recognize alpha- actinin in type IIa or IIb human muscle fibers, but will help detect this protein in type 1 muscle fibers. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-1:100 Immunohistochemistry: 1:40-1:80 for 1 hr. at RT Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: Skeletal muscle Immunohstochemistry (Frozen Unfixed): Normal human striated muscle frozen in isopentane chilled in liquid nitrogen. Staining pattern-striations localized to the Z disc in longitudinal sections. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A0762-01B
Reactivities: Human, Canine, Chicken/Bird, Porcine
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 1ml
Form: Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
P type: Mab
Isotype: IgG1
Purity: Supernatant
References: 1. Johnson M A and Barron M J. Muscle biopsy analysis. Chapter 6. Handbook of muscle disease. Ed R J M Lane. Marcel Dekker. (1996). 2. Wallgren-Pettersson C et al.. Alpha-actinin in nemaline bodies in congenital nemaline myopathy. Neuromusc. Disord. 5:93-104 (1995).
Additional info: Recognizes alpha-actinin in human type 1 slow twitch muscle fibers and on red blood cell membranes. Species Crossreactivity: Chicken, canine and porcine.

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