Actinin, alpha (alpha-Actinin, alpha-Actinin Cytoskeletal Isoform, F-actin Cross-linking Protein, Non-muscle alpha-Actinin)

Alpha-actinin is a cytoskeletal protein located in the Z band/disc region of skeletal muscle where it cross-links with F-actin. The presence of alpha-actinin can be related to a condition known as congenital nemaline myopathy. This antibody does not recognize alpha- actinin in type IIa or IIb human muscle fibers, but will help detect this protein in type 1 muscle fibers.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:50-1:100\nImmunohistochemistry: 1:40-1:80 for 1 hr. at RT\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nWestern Blot: Skeletal muscle\nImmunohstochemistry (Frozen Unfixed): Normal human striated muscle frozen in isopentane chilled in liquid nitrogen. Staining pattern-striations localized to the Z disc in longitudinal sections.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0762-01B

Size

1ml

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Can, Ch/Bird, Prc

Form

Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1. Johnson M A and Barron M J. Muscle biopsy analysis. Chapter 6. Handbook of muscle disease. Ed R J M Lane. Marcel Dekker. (1996). 2. Wallgren-Pettersson C et al.. Alpha-actinin in nemaline bodies in congenital nemaline myopathy. Neuromusc. Disord. 5:93-104 (1995).

Additional Info

Recognizes alpha-actinin in human type 1 slow twitch muscle fibers and on red blood cell membranes. Species Crossreactivity: Chicken, canine and porcine.