Alpha-actinin is a cytoskeletal protein located in the Z band/disc region of skeletal muscle where it cross-links with F-actin. The presence of alpha-actinin can be related to a condition known as congenital nemaline myopathy. This antibody does not recognize alpha- actinin in type IIa or IIb human muscle fibers, but will help detect this protein in type 1 muscle fibers.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:50-1:100
Immunohistochemistry: 1:40-1:80 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.
Positive Control:
Western Blot: Skeletal muscle
Immunohstochemistry (Frozen Unfixed): Normal human striated muscle frozen in isopentane chilled in liquid nitrogen. Staining pattern-striations localized to the Z disc in longitudinal sections.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.