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Activating Transcription Factor 2 (ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558)

Activating Transcription Factor 2 (ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558)

Cat no: A0855-42K

Supplier: United States Biological
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Alternate Name: ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558 Domain Information: basic region leucin zipper Gene Ontology Terms: RNA polymerase II transcription factor activity; transcription co-activator activity Application(s): Suitable for use in ELISA, Western Blot. Other applications not tested. Western blot: Approx 60kD and 28kD bands seen in HeLa cell lysates. Recommended for use at 0.5-2ug/ml. Overnight incubation is recommended with this antibody. Please note that the appox 28kD band we observe maybe explained by the isoform described in Bailey et al [J. Clin. Endocr. Metab. 87: 1717-1728, 2002]. Staining (2ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See A0855-42K-P. Antibody
Catalogue number: A0855-42K
Reactivities: Human, Rat
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: Monzen K, Hiroi Y, Kudoh S, Akazawa H, Oka T, Takimoto E, Hayashi D, Hosoda T, Kawabata M, Miyazono K, Ishii S, Yazaki Y, Nagai R, Komuro I., Smads, TAK1, and their common target ATF-2 play a critical role in cardiomyocyte differentiation., J Cell Biol 2001 May 14;153(4):687-98
Additional info: Recognizes Human, Rat ATF2

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