Activating Transcription Factor 2 (ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558)

Alternate Name: ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558\n\nDomain Information: basic region leucin zipper\n\nGene Ontology Terms: RNA polymerase II transcription factor activity; transcription co-activator activity\n\nApplication(s): Suitable for use in ELISA, Western Blot. Other applications not tested.\nWestern blot: Approx 60kD and 28kD bands seen in HeLa cell lysates. Recommended for use at 0.5-2ug/ml. Overnight incubation is recommended with this antibody. Please note that the appox 28kD band we observe maybe explained by the isoform described in Bailey et al [J. Clin. Endocr. Metab. 87: 1717-1728, 2002].\n\nStaining (2ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See A0855-42K-P. Antibody

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SPECIFICATIONS

Catalog Number

A0855-42K

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Rat

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Monzen K, Hiroi Y, Kudoh S, Akazawa H, Oka T, Takimoto E, Hayashi D, Hosoda T, Kawabata M, Miyazono K, Ishii S, Yazaki Y, Nagai R, Komuro I., Smads, TAK1, and their common target ATF-2 play a critical role in cardiomyocyte differentiation., J Cell Biol 2001 May 14;153(4):687-98

Additional Info

Recognizes Human, Rat ATF2