Home  >  Products  >  Activating Transcription Factor 2 (ATF2, cAMP Response Element Binding Protein 2, CRE BP1, CREB 2, CREB2, CREBP1, HB 16, HB16, MGC111558, mXBP, TREB 7, TREB7)
Activating Transcription Factor 2 (ATF2, cAMP Response Element Binding Protein 2, CRE BP1, CREB 2, CREB2, CREBP1, HB 16, HB16, MGC111558, mXBP, TREB 7, TREB7)

Activating Transcription Factor 2 (ATF2, cAMP Response Element Binding Protein 2, CRE BP1, CREB 2, CREB2, CREBP1, HB 16, HB16, MGC111558, mXBP, TREB 7, TREB7)

Cat no: A0855-42V

Supplier: United States Biological
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Activating Transcription Factor (ATF) Proteins are a family of cellular proteins that contain basic region leucine zipper (bZIP) DNA binding domains. The tax protein of human T cell leukemia virus type-1 (HTLV-1) stimulates in vitro DNA binding of several ATF bZIP domains by enhancing dimerization in absence of DNA resulting in viral transcription and cell transformation. Adenovirus E1a is a protein that stimulates transcription of early viral genes. E1a is not a sequence-specific DNA binding protein and therefore must target the viral promoter through a specific promoter bound protein called Activating Transcription Factor-2. Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1-10ug/ml Western Blot: 1-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A0855-42V
Reactivities: Human
Hosts: Sheep
Applications: Immunoprecipitation, Western Blot
Size: 200ug
Form: Supplied as a liquid in PBS, 0.09% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein G affinity chromatography.
References: 1. Wagner, S. & Green, M. R (1993) HTLV-I Tax protein stimulation of DNA binding of bZIP proteins by enhancing dimerization Science 262: 395 - 359.
Additional info: Recognizes human ATF2. Species sequence homology: mouse, rat, bovine, dog and horse.

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