Home  >  Products  >  Activating Transcription Factor 2 (CT) (ATF2, cAMP Response Element Binding Protein 2, CRE BP1, CREB 2, CREB2, CREBP1, HB 16, HB16, MGC111558, mXBP, TREB 7, TREB7)
Activating Transcription Factor 2 (CT) (ATF2, cAMP Response Element Binding Protein 2, CRE BP1, CREB 2, CREB2, CREBP1, HB 16, HB16, MGC111558, mXBP, TREB 7, TREB7)

Activating Transcription Factor 2 (CT) (ATF2, cAMP Response Element Binding Protein 2, CRE BP1, CREB 2, CREB2, CREBP1, HB 16, HB16, MGC111558, mXBP, TREB 7, TREB7)

Cat no: A0855-43U

Supplier: United States Biological
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The ATF/CREB family of DNA binding proteins consists of transcription factors whose activity is regulated by specific signaling pathways, including the stress/cytokine/radiation inducible pathways that function through SAPK1/JNK activation. Two threonine residues at positions 69 and 71 in the N-terminal region of ATF-2 are essential in mediating adenovirus E1A-inducible transcriptional activation. These residues are efficiently phosphorylated in vivo and are excellent substrates for SAPK1/JNK in vitro. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:10000 Immunohistochemistry (paraffin): 1:250-500 Immunocytochemistry: 1:250-500 Immunoprecipitation: 1:60 Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A0855-43U
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
Additional info: Recognizes human ATF-2. Species Crossreactivity: mouse.

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