Activating Transcription Factor 2, phosphorylated (Ser490/498) (ATF2, cAMP Response Element Binding Protein 2, CRE BP1, CREB 2, CREB2, CREBP1, HB 16, HB16, MGC111558, mXBP, TREB 7, TREB7)

The activating transcription factor ATF2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2. Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF. Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. In addition, ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered an important substrate of signals upstream of the activation of genes associated with neuronal growth and differentiation. ATF expression has also been linked to the depression in humans.\n\nApplications: \nSuitable for use in Dot Blot, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nDot Blot: 1:1000\nWestern Blot: 1:1000\nImmunohistochemistry: 1:1000\nNote: Antibody dilutions and tissue load should be based on tissue type and expected \nphosphorylation state expected expression level.\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A0855-43S

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid (serum)

P Type

Pab

Purity

Serum

References

Bhoumik, A., Jones, N. and Ronai, Z. "Transcriptional switch by activating transcription factor 2-derived peptide sensitizes melanoma cells to apoptosis and inhibits their tumorigenicity," Proc. Natl. Acad. Sci. U.S.A. 101 (2004) 4222 - 4227. Gutpa, S., Campbell, D., Derijard, B. and Davis, R. "Transcription factor ATF2 regulation by the JNK signal transduction pathway," Science 267 (1995) 389 - 393. Ivanov, V.N., Bhoumik, A. and Ronai, Z. "Death receptors and melanoma resistance to apoptosis," Oncogene 22 (2003) 3152 - 3161. Karin, M. and Hunter, T. "Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus," Curr. Biol. 5 (1995) 747 - 757. Laifenfeld, D., Karry, R., Grauer, E., Klein, E. and Ben-Shachar, D. "ATF2, a member of the CREB/ATF family of transcription factors, in chronicstress and consequent to antidepressant treatment: animal models and humanpost-mortem brains," Neuropsychopharmacology 29 (2004) 589 - 97. Maekawa, T., Sakura, H., Kanei-Ishii, C., Sudo, T., Yoshimura, T. and Fujisawa, J. "Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain," EMBO J. 8 (1989) 2023 - 2028.

Additional Info

Specific for ~74kDa human ATF2 protein phosphorylated at Ser490/498. The antibody also recognizes the phosphorylated ~54kDa splice form of ATF2