Activating Transcription Factor 2, phosphorylated (Ser94, Ser112) (ATF2, cAMP Response Element Binding Protein 2, CRE BP1, CREB 2, CREB2, CREBP1, HB 16, HB16, MGC111558, mXBP, TREB 7, TREB7)

The activating transcription factor ATF2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2. Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF2. Serine 490 and serine 498 are novel phosphorylation sites on ATF2 that have recently been identified. In addition, ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered important substrates of signals upstream of the activation of genes associated with neuronal growth and differentiation. ATF2 expression has also been linked to depression in humans. Human ATF2 gene is mapped to chromosome 2q32.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0855-44M

Size

50ug

Applications

IHC

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Karin, M. et al. Curr. Biol. 5:747-757 (1997). 2. Maekawa, T. et al. EMBO J. 8:2023-2028 (1989). 3. Gutpa, S. Science. 267:389-393 (1995). 4. Bhoumik, A. et al. Proc. Natl. Acad. Sci. U.S.A. 101:4222-4227 (2004).

Additional Info

Recognizes human ATF2, phosphorylated at Ser112 or 94. Species Crossreactivity: mouse and rat.