Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, HIGM2) (Control Peptide)

Synthetic peptide corresponding to 14 amino acids near the carboxy-terminus of human AID (GenBank accession no. AAM95402). See corresponding antibody A0855-75J.\n\nActivation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNAediting cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines (1). It is necessary for somatic hypermutation and class switch recombination in B cells (2), but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms (2,3). Although it is structurally and functionally similar to the APOBEC proteins (reviewed in 4), it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G (5).\n\nApplications: \nSuitable for use as a blocking peptide in ELISA, Western Blot with A0855-75J. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: AID peptide is used for blocking the activity of AID antibody (Cat. No. A0855-75J). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37?C.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLiquid peptide is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0855-75J1

Size

50ug

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.

Purity

Highly Purified

References

1. Muramatsu M, Sankaranand VS, Anant S, et al. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA -editing deaminase family in germinal center B cells. J. Biol. Chem. 1999; 274:18470-6.\n2. Muramatsu M, Kinoshita K, Fagarasan S, et al. Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme. Cell 2000; 102:553-63.\n3. Greeve J, Philipsen A, Krause K, et al. Expression of activation-induced cytidine deaminase in human B-cell Hodgkin lymphomas. Blood 2003; 101:3574-80.\n4. Cascalho M. Advantages and disadvantages of cytidine deamination. J. Immunol. 2004; 172:6513-8.\n5. Sheehy AM, Gaddis NC, Choi JD, et al. Isolation of a human gene that inhibits HIV -1 infection and is suppressed by the viral Vif protein. Nature 2002; 418:646-50.\n(06-01D)

Alternative Names

EC=3.5.4.5