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Activation-induced Cytidine Deaminase (AID, AICDA, ARP2, CDA2, HIGM2, Activation-induced Deaminase, Integrated Into Burkitt's Lymphoma Line Ramos)

Cat no: A0855-75E

Activation-induced Cytidine Deaminase (AID, AICDA, ARP2, CDA2, HIGM2, Activation-induced Deaminase, Integrated Into Burkitt's Lymphoma Line Ramos)

Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution: \nELISA: 1:1000\nWestern Blot: 0.1-0.3ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0855-75E

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Crouch EE, Li Z, Takizawa M, Fichtner-Feigl S, Gourzi P, Montano C, Feigenbaum L, Wilson P, Janz S, Papavasiliou FN, Casellas R. Regulation of AID expression in the immune response. J Exp Med. 2007 May 14;204(5):1145-56. Epub 2007 Apr 23.

Additional Info

Recognizes human AID at ~26kD in Human Lymph Node, Spleen and Tonsil lysates (calculated MW of 24.0kD according to NP_065712.1).

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