Activins are dimers consisting of two beta subunits. Several types of beta subunits have been described. The same beta subunits also occur in Inhibins as part of a heterodimer with an alpha subunit. The beta subunits are translated as a precursor form that includes an N-terminal pro region which is proteolytically cleaved to yield the mature C-terminal beta subunit.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human Activin A precursor is ~15ng/well.
Western Blot: 1- 2ug/ml. The detection limit for recombinant human Activin A precursor is ~5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Hybridoma:
Mouse myeloma with B cells obtained from a mouse immunized with a DNA construct encoding the human Activin A pro region fused to mature human Activin beta B chain.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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