Activin B is a TGF-b family member that exhibits a wide range of biological activities including regulation of embryogenesis, osteogenesis, hematopoiesis, reproductive physiology and hormone secretion from the hypothalamic, pituitary and gonadal glands. Activin B, like certain other members of the TGF-b family, signals through the ActRII receptor (Activin Receptor type II). Human Activin B is a 26.0kD disulfide-linked homodimer consisting of two bB chains, each containing 115 amino acid residues.
Applications:
Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1.0ug/ml. The detection limit is ~0.1ng/well.
Western Blot: 1-2ug/ml. The detection limit is ~0.5ng/lane and 1ug/lane under non-reducing and reducing conditions.
Neutralization: ~5-20ug/ml to neutralize 7.5ng/ml of human Activin AB on K562 cells.
Optimal dilutions to be determined by the researcher.
Hybridoma:
Mouse myeloma with B cells obtained from a mouse
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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