Activin Receptor Type IIB (RIIB)

Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated. \n\nApplications: \nSuitable for use in Neutralization, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nNeutralization: ~1-3ug/ml will block 50% of the binding of 30ng/ml of recombinant human Activin A to immobilized recombinant human Act RIIB/Fc Chimera (100ul of a 2ug/ml solution was coated in each well) in an ELISA type assay.\nDirect ELISA: 0.5-1.0ug/ml. The detection limit is ~0.1ng/well.\nWestern Blot: 0.1-0.2ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions.\nImmunohistochemistry (Paraffin): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0856-10A

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose.\nReconstitute with 1ml sterile PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography. \nEndotoxin: <0.1EU/ug (LAL)

Isotype

IgG

Additional Info

Recognizes human Activin Receptor Type IIB (RIIB). Shows <1% crossreactivity with recombinant human Activin RIIA, recombinant human Activin RIA and recombinant human Activin RIB.