ACTL6A, NT (ACTL6A, BAF53, BAF53A, INO80K, Actin-like protein 6A, 53kD BRG1-associated factor A, Actin-related protein Baf53a, ArpNbeta, BRG1-associated factor 53A, INO80 complex subunit K) (Biotin)

This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

031576-Biotin

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Lamesch, P., et al. Genomics 89(3):307-315(2007)\nOlsen, J.V., et al. Cell 127(3):635-648(2006)\nOlsen, J.V., et al. Cell 127(3):635-648(2006)\nCai, Y., et al. J. Biol. Chem. 280(14):13665-13670(2005)\nDoyon, Y., et al. Curr. Opin. Genet. Dev. 14(2):147-154(2004)

Additional Info

Human

Alternative Names

ACTL6A, BAF53, BAF53A, INO80K, Actin-like protein 6A, 53kD BRG1-associated factor A, Actin-related protein Baf53a, ArpNbeta, BRG1-associated factor 53A, INO80 complex subunit K