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ACTN4, NT (Alpha-actinin-4, F-actin Cross-linking Protein, Non-muscle alpha-actinin 4)

Cat no: 029408

ACTN4, NT (Alpha-actinin-4, F-actin Cross-linking Protein, Non-muscle alpha-actinin 4)

Alpha-actinin is a ubiquitously expressed cytoskeletal protein found in numerous actin structures. It consists of an N-terminal actin-binding domain, a central rod domain, and a C-terminal domain; the protein functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins. Alpha-actinin 4 is a non-muscle isoform version of alpha-actinin that is concentrated in the cytoplasm. It plays a role in cell motility, invasion, and metastasis of cancer cells. Alpha-actinin 4 is commonly expressed in filopodia, lamellipodia, stress fibers, and sites of adhesion.\n\nApplications:\nSuitable for use in Immunoprecipitation, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:10,000 \nImmunocytochemistry: 1:100-1:250\nImmunohistochemistry (paraffin): 1:100-1:250\nImmunoprecipitation: 1:10-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman fetal skeletal muscle tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029408

Size

100ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Yl

Additional Info

Recognizes human ACTN4. Species Crossreactivity: Mouse and rat.

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