Acylation Stimulating Protein (ASP)

Acylation Stimulating Protein (ASP, also known as C3a desArg) is one of the activation fragments formed from the activation of a complement cascade. ASP is produced through a process involving three proteins: C3, factor B and adipsin, which are secreted by adipocytes. Interactions of C3 with factor B and with adipsin result in the production of C3a followed by desargination of the carboxyl terminus to generate ASP (C3a desArg). Human ASP contains 77 amino acids with 6 cysteins involved in disulfide bridges between residues 22-49, 23-56 and 36-57. ASP is a highly cationic molecule containing no carbohydrate. ASP has a primary role in the regulation of lipid metabolism in adipocytes, where it stimulates glucose uptake, increases the activity of diacylglycerol acyltransferase, and inhibits hormone-sensitive lipase activity. In cellular studies, ASP increases fat storage through increased triglyceride synthesis and decreased intracellular lipolysis. In animal models, ASP-deficient mice demonstrate reduced body weight, reduced leptin and reduced adipose tissue mass. ASP deficiency also results in protection against development of obesity. In humans, a number of studies have shown the relationship between ASP, obesity, diabetes and dyslipidemia. It was reported that the concentration of circulating ASP is positively related to body adiposity and decreases after weight loss. Because ASP enhances triglyceride storage, while interfering with ASP production reduces body fat and protects against diet-induced obesity and insulin resistance, reduction of the production of ASP and ASP receptor antagonists represents a potential approach for treating obesity and type 2 diabetes.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0858-01A

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from 0.05M Phosphate buffer, 0.1M sodium chloride, pH 7.2. No Preservative added. Reconstitute in 100ul of sterile dH2O.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Cianflone K, Phelis S, Davignon J, Gilfix BM.: ApoE phenotype influences plasma ASP in hyperapoB subjects. Atherosclerosis. Oct;170(2):285-91 (2003) 2. Faraj M, Havel PJ, Phelis S, Blank D, Sniderman AD, Cianflone K.: Plasma acylation-stimulating protein, adiponectin, leptin, and ghrelin before and after weight loss induced by gastric bypass surgery in morbidly obese subjects. J Clin Endocrinol Metab. Apr;88(4):1594-602 (2003) 3. Matthan NR, Cianflone K, Lichtenstein AH, Ausman LM, Jauhiainen M, Jones PJ.: Hydrogenated fat consumption affects acylation-stimulating protein levels and cholesterol esterification rates in moderately hypercholesterolemic women. J Lipid Res. Nov;42(11):1841-8 (2001) 4. Faraj M, Jones P, Sniderman AD, Cianflone K.: Enhanced dietary fat clearance in postobese women. J Lipid Res. Apr;42(4):571-80 (2001) 5. Kalant D, Phelis S, Fielding BA, Frayn KN, Cianflone K, Sniderman AD.: Increased postprandial fatty acid trapping in subcutaneous adipose tissue in obese women. J Lipid Res. Dec;41(12):1963-8 (2000) 6. Murray I, Sniderman AD, Cianflone K.: Enhanced triglyceride clearance with intraperitoneal human acylation stimulating protein in C57BL/6 mice. Am J Physiol. Sep;277(3 Pt 1):E474-80 (1999) 7. Saleh J, Summers LK, Cianflone K, Fielding BA, Sniderman AD, Frayn KN.: Coordinated release of acylation stimulating protein (ASP) and triacylglycerol clearance by human adipose tissue in vivo in the postprandial period. J Lipid Res. Apr;39(4):884-91 (1998) 8. Cianflone K.: The acylation stimulating protein pathway: clinical implications. Clin Biochem.Jun;30(4):301-12 (1997)

Additional Info

Recognizes human Acylation Stimulating Protein (ASP).