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ADAM 10 (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 10)

Cat no: A0859-20

ADAM 10 (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 10)

ADAM10 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It cleaves the membrane-bound precursor of TNF-alpha at 76-Ala-|-Val-77 to its mature soluble form. It is responsible for the proteolytic release of several cell-surface proteins, including heparin binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). It contributes to the normal cleavage of the cellular prion protein and is involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicules, suggesting a vesicle-based protease activity.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 1:500-1:2000 detected ADAM 10 on 10ug of RAW264.7 lysates. RAW264.7 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with A0859-20 (1:1000). Proteins were visualized using an IgG (HRP) donkey anti-rabbit and chemiluminesence detection system. \nImmunohistochemistry: A previous lot of this antibody was used to detect ADAM10 in formalin-fixed, paraffin-embedded renal tissue and renal cell carcinoma tissue (Gutwein, P., et al., 2009).\nImmunofluorescence: A previous lot of this antibody was used to detect ADAM10 in RCC4 and A498 cells (Gutwein, P., et al., 2009).\nOptimal dilutions to be determined by the researcher.\n \nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0859-20

Size

100ug

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Bov

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Gutwein, P., et al. EUROPEAN JOURNAL OF CANCER. 45(2009):478

Additional Info

Recognizes human ADAM 10, C-terminus. Species Crossreactivity: bovine and mouse

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