ADAM12 (A disintegrin and metalloprotease domain 12; also meltrin-a) is a
120kD type I transmembrane glycoprotein member of the metzincin family of
proteases. It is synthesized as a 909 amino acid (aa) preproprecursor that
contains a 25kD proregion (aa 29-207) and a 92kD mature molecule.
Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface
expression. A 68kD soluble splice form exists in pregnancy where 36 aa
replace the C-terminal 205 aa (705-909). This circulating ADAM12 isoform
remains associated with its prodomain via noncovalent linkage, possibly acting
as an enzyme regulator. Over aa 29-513, human ADAM12 shares 78% and
79% aa identity with bovine and mouse ADAM12, respectively.
Applications:
Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 0.1- 0.2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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