ADAM 17, CT (ADAM17, A Disintegrin and Metalloproteinase Domain 17 (Tumor Necrosis Factor, alpha, Converting Enzyme), CSVP, TACE, cSVP, CD156b, CD156b, MGC71942, TACE, cSVPADAM Metallopeptidase Domain 17, TNF-alpha Converting Enzyme, A Disintegrin and Met

LQRQNRVDSKETEC, C Terminus\nControl peptide corresponding to polyclonal antibody, Catalog#A0859-35H, goat x human.\n\nAlternate Name: ADAM17, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), CSVP, TACE, cSVP, CD156b, CD156b, MGC71942, TACE, cSVPADAM metallopeptidase domain 17, TNF-alpha converting enzyme, a disintegrin and metalloproteinase domain 17, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), snake venom-like protease\n\nApplication(s):\nSuitable for use in Peptide ELISA. Other applications have not been tested. \n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking (see corresponding antibody A0859-35H):\nAntibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

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SPECIFICATIONS

Catalog Number

A0859-35H-P

Size

100ug

Applications

ELISA

Form

Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O.

Purity

Highly Purified

References

Black RA, Rauch CT, Kozlosky CJ, Peschon JJ, Slack JL, Wolfson MF, Castner BJ, Stocking KL, Reddy P, Srinivasan S, Nelson N, Boiani N, Schooley KA, Gerhart M, Davis R, Fitzner JN, Johnson RS, Paxton RJ, March CJ, Cerretti DP, A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells, Nature. 1997 Feb 20;385(6618):729-33