ADAM19 (a disintegrin and metalloprotease 19; also MADDAM and Meltrin-b) is a
95-100kD member of the M12B peptidase family of enzymes. It is expressed on
multiple cell types including monocytes, fibroblasts, osteoblasts and dendritic cells. The
human ADAM19 proenzyme is a 931 amino acid (aa) type I transmembrane protein. It
contains a 178 aa cleavable proregion (aa 26-203) and a 497 aa extracellular domain
(ECD) (aa 204-702). The ECD contains a metalloprotease domain (aa 211-409), a
disintegrin region (aa 417-503) and a Cys-rich domain (aa 504-650). The ECD
circulates in plasma bound to 700kD a2-Macroglobulin. One splice variant shows an
alternate start site at Met71, while two isoforms show variations afterLys902. Over
aa 205-702, human ADAM19 is 87% aa identical to mouse ADAM19.
Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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