ADAM23 (a disintegrin and metalloprotease domain 23; also MDC3) is a 100kD member of the M12B peptidase family of enzymes. It is expressed on fetal neurons in the hippocampus and cerebellum and serves as a counter-receptor for alpha v beta 3 integrin. The human ADAM23 proprecursor is a 773aa type I transmembrane (TM) protein. It contains a 227aa cleavable proregion (aa60-286) and a 506aa extracellular domain (ECD) (aa287-792) that is part of a 70kD mature molecule. The ECD contains a nonfunctional metalloprotease domain (aa299- 494), an integrin-binding disintegrin region (aa511-585) and a Cys-rich domain (aa589-611).
Two splice variants exist. One shows an in-frame 46aa substitution for aa787-832 that generates a soluble form, while a second shows an in-frame 31aa TM substitution for aa787-817. Over aa 1-585, human ADAM23 is 92% aa identical to mouse ADAM23.
Applications:
Suitable for use in Neutralization, RIA, Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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