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ADAM 9 (A Disintegrin and Metalloproteinase Domain 9, ADAM Metallopeptidase Domain 9, ADAM9, ADAM-9, Cellular Disintegrin-related Protein, Disintegrin and Metalloproteinase Domain-containing Protein 9, KIAA0021, MDC9, Meltrin gamma, MLTNg, Metalloprotease

Cat no: A0859-10F

ADAM 9 (A Disintegrin and Metalloproteinase Domain 9, ADAM Metallopeptidase Domain 9, ADAM9, ADAM-9, Cellular Disintegrin-related Protein, Disintegrin and Metalloproteinase Domain-containing Protein 9, KIAA0021, MDC9, Meltrin gamma, MLTNg, Metalloprotease

The ADAM (A Disintegrin and A Metalloprotease) family of multidomain membrane proteins influences cell signaling and adhesion by shedding cell surface proteins such as cytokines and growth factors, by influencing cell adhesion to the extracellular matrix (ECM), and by directly remodeling the ECM. Conserved domains in ADAM family members include a prodomain, a zinc-dependent metalloprotease domain, a disintegrin domain, a cysteine-rich domain, an EGF-like sequence and a short cytoplasmic tail (1,2).The prodomain is thought to aid in protein folding. Disintegrin and cysteine-rich domains mediate adhesion at least in part through binding to integrins. Phosphorylation of the cytoplasmic tail as well as its interaction with other signaling proteins may influence intra- and extracellular signaling (1). ADAM9 is widely distributed and has been shown to affect migration in skin keratinocytes (3,4). ADAM9 is overexpressed in prostate cancer (5), pancreatic cancer (6), gastric cancer (7) and has been linked to invasion and metastasis in small cell lung cancer (8). An alternatively spliced short (50 kDa) form of ADAM9 containing protease activity is involved in tumor cell invasion (9).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0859-10F

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

Additional Info

Recognizes endogenous levels of total human ADAM9 protein. Species crossreactivity: Mouse, rat and monkey.

Alternative Names

EC=3.4.24.-, CORD9, Meltrin-gamma, Metalloprotease/disintegrin/cysteine-rich Protein 9

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