ADAM10 (Disintegrin and Metalloproteinase Domain-containing Protein 10, ADAM 10, CDw156, Kuzbanian Protein Homolog, Mammalian Disintegrin-metalloprotease, CD156c, KUZ, MADM)

Proinflammatory cytokine tumor necrosis factor-a? (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch, and its ligand delta, are all membrane-bound molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ) and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:250-1:1000\nELISA: 1:5000-1:20,000\nWestern Blot: 1:500-1:2000\nImmunohistochemistry: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

168008

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.

P Type

Pab

Purity

Purified from monospecific antiserum by DEAE chromatography.

Isotype

IgG

Additional Info

Recognizes human ADAM10. Species sequence homology: bovine and rat.

Alternative Names

A disintegrin and metalloprotease domain 10 antibody, A Disintegrin And Metalloproteinase Domain 10 antibody, ADAM 10 antibody, ADAM metallopeptidase domain 10 antibody, CD 156c antibod, KUZ, MADM