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ADAM12 (ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, A disintegrin and metalloproteinase domain 12, Meltrin alpha)

Cat no: A0859-25Q1

ADAM12 (ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, A disintegrin and metalloproteinase domain 12, Meltrin alpha)

ADAM12 is a 120kD type I transmembrane glycoprotein member of the metzincin family of proteases. It is synthesized as a 909aa preproprecursor that contains a 25kD proregion aa29-207 and a 92kD mature molecule. Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression. A 68kD soluble splice form exists in pregnancy where 36aa replace the C-terminal 205 aa705-909. This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator. Over aa29-513, human ADAM12 shares 78-79aa identity with bovine and mouse ADAM12, respectively.\n\nApplications:\nSuitable for use in Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunoprecipitation: 25ug/ml detects ADAM12 in conditioned cell culture medium spiked with recombinant human ADAM12.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0859-25Q1

Size

100ug

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human ADAM12. Does not react with recombinant human ADAM8, 9, 10, 15, 17, 19, 22, 23, 32 or 33.

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