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ADAM12 (MCMP, MLTN, MLTNA, MCMPMltna, Meltrin alpha, A disintegrin and metalloproteinase domain 12) (Biotin)

Cat no: A0859-25J

ADAM12 (MCMP, MLTN, MLTNA, MCMPMltna, Meltrin alpha, A disintegrin and metalloproteinase domain 12) (Biotin)

ADAM12 (A disintegrin and metalloprotease domain 12; also meltrin-alpha) is a 120kD type I transmembrane glycoprotein member of the metzincin family of proteases. It is synthesized as a 909aa preproprecursor that contains a 25kD proregion (aa29-207) and a 92kD mature molecule. Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression. A 68kD soluble splice form exists in pregnancy where 36aa replace the C-terminal 205aa (705-909). This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator. Over aa29-513, human ADAM12 shares 78% and 79% aa identity with bovine and mouse ADAM12, respectively. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-0.2ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0859-25J

Size

50ug

Applications

WB

Hosts

Sheep

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with BSA. Reconstitute with 40-50% glycerol, PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human ADAM12.

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