Home  >  Products  >  ADAM15 (a Disintegrin and Metalloprotease 15, Metargidin, MDC15)
ADAM15 (a Disintegrin and Metalloprotease 15, Metargidin, MDC15)

ADAM15 (a Disintegrin and Metalloprotease 15, Metargidin, MDC15)

Cat no: A0859-31B

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry and Direct ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction (same/less than)200ul. Immunocytochemistry: 10ug/ml. Cells fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100 and 1% BSA. After blocking, cells were incubated with diluted A0859-31B followed by labeled anti-mouse IgG. Western Blot: 1-2ug/ml. Detects ~5ng/lane of rhADAM15 under non-reducing conditions. Immunoprecipitation: 25ug/ml Direct ELISA: 1ug/ml detects ~0.3ng rhADAM15/well. Optimal dilutions to be determined by the researcher. Positive Control: MCF-7 cells Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A0859-31B
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Size: 500ug
Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes human ADAM15 ectodomain. Cross-reactivity: Shows 100% with rmADAM15 in Direct ELISA. Does not cross-react with rmADAM15, 10 , 28 and rhADAM8, 9, and 17 (TACE) in Western Blot. Does not cross-react with rhADAM8, 9,17, 28 and rmADAM9 and 10 in Direct ELISA.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave