ADAMTS1 (C3-C5, KIAA1346, METH1, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1)

ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. Scott (2000) mapped the ADAMTS1 gene to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. Kuno et al. (1997) found that mouse Adamts1 expression could be induced in vitro in colon adenocarcinoma cells by stimulation with the inflammatory cytokine interleukin 1-alpha, or in vivo in kidney and heart by intravenous administration of lipopolysaccharide. Vazquez et al. (1999) determined that ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.\n\nUniProt Number:\nP97857\n\nGene ID:\nADAMTS1\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144128

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kuno, K., Kanada, N., Nakashima, E., Fujiki, F., Ichimura, F., Matsushima, K. Molecular cloning of a gene encoding a new type of metalloproteinase-disintegrin family protein with thrombospondin motifs as an inflammation associated gene. J. Biol. Chem. 272: 556-562, 1997.\n2. Scott, A. F. Personal Communication. Baltimore, Md. 9/18/2000.\n3. Vazquez, F., Hastings, G., Ortega, M.-A., Lane, T. F., Oikemus, S., Lombardo, M., Iruela-Arispe, M. L. METH-1, a human ortholog of ADAMTS-1, and METH-2 are members of a new family of proteins with angio-inhibitory activity. J. Biol. Chem. 274: 23349-23357, 1999.

Additional Info

Recognizes mouse and rat ADAMTS1. No crossreactivity with other proteins.