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ADAMTS13 (A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13)

Cat no: A0859-61F1

ADAMTS13 (A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13)

ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin motifs 13) is a secreted multi-domain zinc protease primarily produced by the liver. In the circulation, it is responsible for cleaving von Willebrand factor (vWF) between Tyr842 and Met843. Defects in ADAMTS13 activity result in the generation of ultra large vWF, leading to thrombotic thrombocytopenic purpura (TTP). The amino acid sequence of human ADAMTS13 in the region of aa34-688 is 76% identical to that of mouse/rat. \n\nApplications: \nSuitable for use in Flow Cytometry, Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry (Intercellular): 2.5ug labels 10e6 HUVEC human umbilical vein endothelial cells fixed with paraformaldehyde and permeabilized with saponin\nWestern Blot: 1ug/ml using recombinant human ADAMTS13 under non-reducing conditions only. \nImmunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human full length ADAMRT13 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0859-61F1

Size

100ug

Applications

ELISA, FC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human ADAMTS13. Does not crossreact with recombinant human ADAMTS1, ADAMTS4, ADAMTS5 or ADAMTS-L1.2.

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