ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motifs 4)

ADAMTS4 is a member of the family of secreted zinc proteases with a multi-domain structure (1-3). The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich and spacer. It is the only ADAMTS identified that has one TS type I motif. It is an active protease effectively cleaving alpha-2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4-6). It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7). However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8). The purified recombinant human ADAMTS4 starts at the catalytic domain and ends before the spacer domain. If desired, the aggrecanase activity can be inhibited by 5mM 1,10-phenanthroline and recombinant human TIMP-3.\n\nApplications: \nSuitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.1ug/ml\nImmunoprecipitation: 25ug/ml immunoprecipitates ADAMTS4 from conditioned cell culture medium spiked with recombinant human ADAMTS4.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0859-60B

Size

100ug

Applications

ELISA, IP, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography

Isotype

IgG

References

1. Totorella, M. D. et al. (1999) Science 284:1664. 2. Porter, S. et al. (2005) Biochem. J. 386:15. 3. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94. 4. Tortorella, M. D. et al. (2004) J. Biol. Chem. 279:17554. 5. Struglics, A. et al. (2006) Osteoarthritis Cartilage. 14:101. 6. Kashiwagi, M. et al. (2001) J. Biol. Chem. 276:12501. 7. Pratta, M. A. et al. (2003) Arthritis Reum. 48:119. 8. Glasson, S. S. et al. (2004) Arthritis Reum. 50:2547.

Additional Info

Recognizes human ADAMTS4. Shows ~10% crossreactivity with recombinant human ADAMTS5 and <1% crossreactivity with recombinant human ADAMTS13, ADAMTS