ADAP (Adhesion and Degranulation-promoting Adaptor Protein, SLAP-130, FYB)

ADAP (adhesion and degranulation-promoting adaptor protein/SLAP-130/Fyb) is an SH3 domain-containing adaptor protein expressed by T cells, NK cells, and myeloid cells (1,2). There are two isoforms of ADAP with predicted molecular weights of 85 kDa and 90 kDa, but observed molecular weights of 120 kDa and 130 kDa (1-3). ADAP was identified as an adaptor protein that interacts with SLP-76 following T cell receptor (TCR) stimulation and was subsequently found to be important for several aspects of T cell activation (1,2). For example, ADAP is required for integrin-dependent clustering, signaling, and adhesion (4,5). In addition, ADAP interacts with CARMA1 and facilitates assembly of the CARMA1-Bcl10-MALT1 complex important for NF-kB activation downstream of TCR activation (6). Finally, following binding of a T cell to an antigen presenting cell, ADAP forms a ring at the immunological synapse that recruits dynein to enable microtubule-organizing center polarization (7).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137405

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Musci, M.A. et al. (1997) J Biol Chem 272, 11674-7. 2. Schraven, B. et al. (1997) Immunol Lett 57, 165-9. 3. Veale, M. et al. (1999) J Biol Chem 274, 28427-35. 4. Griffiths, E.K. et al. (2001) Science 293, 2260-3. 5. Peterson, E.J. et al. (2001) Science 293, 2263-5. 6. Medeiros, R.B. et al. (2007) Science 316, 754-8. 7. Combs, J. et al. (2006) Proc Natl Acad Sci U S A 103, 14883-8.

Additional Info

Recognizes endogenous levels of total human ADAP. Species Crossreactivity: mouse.