ADAP (SLAP-130, Fyb)

Human SH2 domain containing leukocyte protein-76-Associated Phosphoprotein of 130kD (SLAP-130), also known as Fyb-120 and ADAP, is an 86kD, 783 amino acid, cytoplasmic protein that runs anomalously as a 120-130kD protein in SDS-PAGE. Human SLAP-130 is a hematopoietic-specific adaptor which associates with other cytoplasmic proteins such as SH2-containing Leukocyte Phosphoprotein of 76kD (SLP-76). Adaptors integrate signals from surface receptors and are essential components of many immune cell-signaling cascades. There is at least one known 46 aa longer alternatively-spliced SLAP-130 isoform. Over the region used as immunogen, SLAP-130 is 66% and 76% identical to the corresponding canine and mouse proteins, respectively. \n \nApplications:\nWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human SLAP-130. The detection limit for rhSLAP-130 is approximately 1ng/lane under non-reducing and reducing conditions. \nDirect ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human SLAP-130. The detection limit for rhSLAP-130 is approximately 0.2ng/well. \nOptimal dilutions should be determined by each laboratory for each application. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS, with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0859-43A

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

P Type

Pab

Purity

Purified by human SLAP-130 affinity chromatography.

Isotype

IgG

Additional Info

Selected for its ability to recognize human SLAP-130 in direct ELISAs and Western Blots.