ADAP (SLAP-130, Fyb)

FYN-binding protein (FYB) is an adapter protein for Fyn and SLP-76 signaling cascades in T cells. A hematopoietic protein, FYB associates with p59fyn and becomes tyrosine-phosphorylated in response to receptor ligation. FYB has been associated to modulation of IL-2 expression in T cells and platelet activation (3). Fyn (p59Fyn) is a 59kDa member of the Src family of tyrosine kinases. The carboxy terminus of Fyn shares extensive amino acid sequence homology with Src, but is very different within the amino-terminal 81 amino acid residues. The Fyn protein is synthesized and N-myristoylated on cytosolic polysomes and then rapidly targeted to the plasma membrane, where it is palmitoylated. Fyn's biological functions are diverse and include signaling via the T cell receptor, regulation of brain function and adhesion mediated signaling (2, 3). FYN-binding protein (FYB) is an adapter protein for Fyn and SLP-76 signaling cascades in T cells. A hematopoietic protein, FYB associates with p59fyn and becomes tyrosine-phosphorylated in response to receptor ligation. FYB has been associated to modulation of IL-2 expression in T cells and platelet activation (3).\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500 \nImmunohistochemistry: 1:100-250 \nImmunocytochemistry: 1:100-250 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

A0859-43E

Size

100ul

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Renzoni D.A., et al; Biochemistry 35:15646-1565, 1996. 2. Mustelin Tet al; Eur J Immunol. 1992 May;22(5):1173-8. 3. Silva AJ, et al. PNAS USA, 94(14):7493-7498, 1997.

Additional Info

Recognizes human FYB