ADAP (SLAP-130, Fyb, FYN Binding Protein, Adhesion and Degranulation Promoting Adaptor Protein, B630013F22Rik, FYB-120/130, FYN-binding Protein, FYN-T-binding Protein, p120/p130, SLP-76-associated Phosphoprotein)

FYB (FYN binding protein) was initially identified as a molecular adaptor that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of FYB, ultimately activates the NFkB family of transcription factors. More recent experiments have shown that FYB controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bclx(L), suggesting that FYB regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of FYB are known to exist.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.5-2.0ug/ml detected ADAP/SLAP-130/Fyb in RIPA lysates from RAW 264.7 macrophage cells. RAW 264.7 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0859-43. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.\nImmunoprecipitation: 4ug immunoprecipitated ADAP/SLAP-130/Fyb from 500ug of RAW 264.7 macrophage RIPA lysate.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-40J: IgG, Fc (HRP) Pab Gt x Rb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0859-43

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Liping, G., et al., Proc. Natl. Acad. Sci. 98: 11,527-11,532 (2001). 2. Boerth, N.J., et al., J. Biol. Chem. 275: 5143-5152 (2000). 3. Hunter, A.J., et al., J. Immunol. 164: 1143-1147 (2000).

Additional Info

Recognizes mouse ADAP/SLAP-130/Fyb, Mr ~130kD. An unknown protein was also detected, Mr ~40kD that is also inhibited by the immunizing peptide. Species Sequence Homology: human and rat.