Adaptin, alpha

Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Src homologous and collagen-like (Shc) and epidermal growth factor (EGF) receptor. AP2 is composed of four subunits, two separate 100kD gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17kD subunit. There are two alpha-adaptin genes, alphaA and alphaC which have a tissue specific pattern of expression. \n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:100; Recognizes an ~100kD doublet representing the two isoforms of alpha-adaptin from porcine brain extract. \nImmunohistochemistry: paraffin sections\nImmunofluorescence (IC): 1:100; Staining of alpha-adaptin in transfected COS cells yields distinct punctate dots on the plasma membrane.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0859-90

Size

500ul

Applications

IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, Prc

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG2a

References

General: This antibody has been used in: J. Cell Biol., 104: 887-895, 1987.General:Cell, 94: 131-141, 1998.EMBO J., 12(5): 2169-2180, 1993.J. Biol. Chem., 271(9): 5265-5269, 1996.J. Cell Biol., 117(6): 1171-1179, 1992.J. Cell Biol., 131(6): 1831-1838, 1995.J. Cell Science, 108: 2865-2875, 1995.J. Cell. Biol., 136(4): 811-821, 1997.Neuron, 16: 481-486, 1996.Science, 261: 612-615, 1993.

Additional Info

Recognizes bovine alpha Adaptin. Detects the products of both alpha-adaptin genes, alpha A and alpha C, as well as an alternatively spliced isoform of alpha A found in neurons. \nSpecies Crossreactivity: human, rat, mouse, porcine, chicken, primate and amphibians.