Adaptor Protein, Phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1, DIP13alpha, Signaling Adaptor Protein DIP13alpha, adaptor protein containing pH Domain, PTB domain and leucine zipper motif)

The APPL1 multidomain adaptor protein is a BAR-domain protein family member that is involved in membrane trafficking within a number of signal transduction pathways (1). The N-terminal BAR domain mediates the for- mation of crescent-shaped APPL1 homodimers (or APPL1 and APPL2 heterodimers) that are important in lipid binding and membrane curvature sensing (1). The PH domain of APPL1 is required for binding of the adaptor protein to Rab5 GTPase (2). In response to extracellular stimuli, Rab5 GTP hydrolysis releases APPL1 from the endosome and allows translocation of APPL1 to the nucleus where it joins a protein complex that controls chromatin remodeling and gene expression (3). The C-terminal PTB domain of APPL1 enables an interaction between APPL1 and the TrkA neuro- trophin receptor. An association between these two proteins and the TrkA-interacting protein GIPC1 within endosomes is required for nerve growth factor (NGF) mediated signaling (4). APPL1 also binds follicle-stimulating hormone (FSH) receptors, which may provide a relay of FSH signaling to the PI3K/Akt pathway (5). The APPL1 adaptor protein is im- plicated in insulin signaling, as interaction between APPL1 and Akt2 is required for insulin-stimulated translocation of GLUT4 receptor proteins. Both induced overexpression and knockdown of APPL1 inhibit insulin

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SPECIFICATIONS

Catalog Number

A0859-44B

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

(1) Habermann, B. (2004) EMBO Rep 5, 250-5. (2) Zhu, G. et al. (2007) EMBO J 26, 3484-93. (3) Miaczynska, M. et al. (2004) Cell 116, 445-56. (4) Lin, D.C. et al. (2006) Mol Cell Biol 26, 8928-41. (5) Nechamen, C.A. et al. (2004) Biol Reprod 71, 629-36. (6) Saito, T. et al. (2007) J Biol Chem 282, 32280-7. (7) Mao, X. et al. (2006) Nat Cell Biol 8, 516-23. (8) Cheng, K.K. et al. (2007) Diabetes 56, 1387-94. (9) Liu, J. et al. (2002) J Biol Chem 277, 26281-5.

Additional Info

Detects endogenous levels of total huamn APPL1 protein.