Adaptor Protein, Phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1, DIP13alpha, Signaling Adaptor Protein DIP13alpha, adaptor protein containing pH Domain, PTB domain and leucine zipper motif)

Cat no: A0859-44C

Supplier: United States Biological

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Additional Information

APPL1, an adaptor protein containing an NH2-terminal Bin/Amphiphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain and a COOH-terminal phosphotyrosine binding (PTB) domain, was originally identified as an interacting partner of Akt in a yeast two-hybrid assay using Akt2 as a bait. APPL1 binds to a number of cell surface receptors (TrkA, DCC, adiponectin, FSH) and intracellular signaling molecules (small GTPase Rab5, GIPC and inositol 5- phosphatase, suggesting that APPL1 may act as a common relay to coordinate diverse signaling cascades. APPL1 potentiates insulin-mediated Akt activation by counteracting the effect of the Akt inhibitor TRB3. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA:0.5-1ug/ml Western Blot:0.1-0.2ug/ml Immunohistochemistry:1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	A0859-44C
Reactivities:	Human, Mouse, Rat, Non-Human Primate
Hosts:	Rabbit
Applications:	ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Size:	100ug
Form:	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA.
P type:	Pab
Isotype:	IgG
Purity:	Purified by immunoaffinity chromatography.
Additional info:	Recognizes human APPL1. Species Cross-Reactivity: monkey, mouse, rat.

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Adaptor Protein, Phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1, DIP13alpha, Signaling Adaptor Protein DIP13alpha, adaptor protein containing pH Domain, PTB domain and leucine zipper motif)

Cat no: A0859-44C

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