Home  >  Products  >  Adducin, alpha, phosphorylated (Ser726) (Alpha-adducin, Erythrocyte Adducin Subunit alpha, ADD1, ADDA)
Adducin, alpha, phosphorylated (Ser726) (Alpha-adducin, Erythrocyte Adducin Subunit alpha, ADD1, ADDA)

Adducin, alpha, phosphorylated (Ser726) (Alpha-adducin, Erythrocyte Adducin Subunit alpha, ADD1, ADDA)

Cat no: A0859-55L

Supplier: United States Biological
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Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes. Adducin is a heterodimeric protein that consists of related subunits; alpha and beta, or alpha and gamma subunits. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot:1:500-1:1,000 Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0859-55L
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human ADD1 when phosphorylated ar (Ser726). Species Cross-Reactivity: mouse, rat.

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