Adenosine A2a Receptor

The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis. Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs). Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b , and A3. The high affinity receptor A1 inhibits adenyl cyclase, whereas low affinity receptor A2a stimulates the cyclase via G proteins. A2a receptor is believed to cause vasorelaxation in coronary artery. Various ARs and their subtypes have recently been cloned from several species. ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains. The N-termini are predicted to be extracellular and the C-termini cytoplasmic. There is an overall 30% homology among the four ARs. ARs are distributed throughout the body. \n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution: \nELISA: 1:10,000-1:50,000\nImmunohistochemistry (Frozen): 10-50ug/ml \nWestern Blot: 1-10ug/ml (Chemiluminescence technique), 40-100ug membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0869-58

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Rat, Bov, Can, Prc

Form

Supplied as a liquid in PBS ,pH 7.4, 0.1% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Science 244: 569-572 (1989). 2. Ann. Rev. Pharmacol. 35: 581-606 (1995). 3. FASEB J. 8: A850 (1994). 4. Cardiovascular Res. 27: 62-67 (1993). 5. Marala, R.B. and Mustafa, S.J., J. Pharmacol. Exp. Ther. 286: 1051-1057 (1998).

Additional Info

Recognizes canine Adenosine A2a receptor. Recognizes a band of ~45kD in human (coronary artery), and rat, porcine and bovine striatal membranes. Species Crossreactivty: human, rat, bovine, porcine.