Adenosine A3 Receptor (A3AR)

The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis. Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs). Based upon biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b and A3. The high affinity receptor A1 inhibits adenyl cyclase via G proteins. A2a receptor is believed to cause vasorelaxation in the coronary artery. Various ARs and their subtypes have recently been cloned from several species. ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains. The N-termini and C-termini are predicted to be extracellular and cytoplasmic, respectively. There is an overall 30% homology among the four ARs. ARs are distributed throughout the body. Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 (Chromosome 1).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000\n\nELISA: 1:10,000-1:50,000 using 50-100ng A3R control peptide per well.\n\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: A0869-70G\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A0869-70D

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Zhou, Q-Y., et al., Proc. Natl. Acad. Sci. 89: 7,432-7,436 (1992). 2. Tucker, A.L. and J. Linden, Cardiovascular Res. 27: 62-67 (1993). 3. Olah, M.E. and G. Stile, Ann. Rev. Pharmacol. 35: 581-606 (1995). 4. Linden, J., et al., Mol. Pharmacol. 44: 524-532 (1993). 5. Sajjadi, F.G., et al., BBA 1179: 105-107 (1993).

Additional Info

Recognizes Adenosine A3 Receptor. Species crossreactivity: The peptide used for making antibodies to rat A3R shows 46% homology (7/15 AA) with human and 20% (3/15 AA) with sheep A3R sequences. Antibody crossreactivity in various species is not established.