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Adenosine Deaminase (ADA, Adenosine Aminohydrolase)

Adenosine Deaminase (ADA, Adenosine Aminohydrolase)

Cat no: A0870-07C

Supplier: United States Biological
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ADA is an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this protein and have been linked to human diseases. Deficiency in ADA causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia (1). Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:10,000 Immunocytochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: A0870-07C
Reactivities: Human
Hosts: Rabbit
Applications: Immunocytochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
P type: Mab
Purity: Supernatant
References: 1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1; 33: D54-8.
Additional info: Recognizes human ADA at ~41kD.

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