Adenosine Receptor A2A

Adenosine receptors, a class of purinergic G-protein coupled receptors with adenosine as endogenous ligands mediate several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The activity of A2A adenosine receptor is mediated by G proteins which activate adenylyl cyclase. It is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. Adenosine receptor A2A-R contributes to motoneuron survival by transactivating the tyrosine kinase receptor TrkB and may suggest a new strategy for using this mechanism in neurodegenerative diseases.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:5000. Detects Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras.\nImmunohistochemistry: 1ug/ml. Detects Adenosine Receptor A2A in paraformaldehyde-fixed mouse brain striatal sections. Not detected in Adenosine Receptor A2A knockout mice.\nFlow Cytometry: Detects Adenosine Receptor A2A in transfected HEK293 cells.\nOptimal dilutions to be determined by the researcher. \n\nPositive Control: \nMouse brain striatal sections\n\nRecommended Secondary Antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo\nI1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0869-59

Size

200ug

Applications

FC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, GP, Rabbit

Form

Supplied as a liquid in 0.01M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Rosin, D.L., et al., J. Comp. Neurol. 401: 163-186 (1998). 2. Hettinger, B.D., et al., J. Comp. Neurol. 431: 331-346 (2001). 3. Koshiba, M., et al., Mol. Pharmacol. 55: 614-624 (1999).

Additional Info

Recognizes human adenosine receptor A2A at ~43kD. \nSpecies Crossreactivity: mouse, rat, rabbit and canine. Crossreactivity with guinea pig also reported.