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Adenovirus Fiber (Monomer and Trimer) (BSA & Azide Free)

Cat no: A0880-19AX

Adenovirus Fiber (Monomer and Trimer) (BSA & Azide Free)

Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta-sheet and beta-bends, and a knob at the C-terminus that contains the typespecific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the viron. \n\nApplications:\nSuitable for use in Immunofluorescence and Western Blot. Other applications have not been tested.\n\nRecommended Dilution: \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nAdenovirus infected cells and tissues. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0880-19AX

Size

100ug

Applications

IF, WB

Hosts

Mouse

Form

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See A0880-19A

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

Key References: 1. Hong, J.S., Engler, J.A. Virology., 185, 758

Additional Info

Recognizes both monomers and trimers, as judged by reactivity with both boiled (monomers (62kD) only) and unboiled (both monomers and trimers (180

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