Adenovirus, Type 5, Early Region (E1A)

The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46kD) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen. \n\nApplications:\nSuitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions: \nImmunofluorescence: 0.2-0.4ug/ml\nImmunoprecipitation (Native): 2ug/mg protein lysate. Use Protein A. \nWestern Blot: 0.5-1ug/ml for 2 hours at RT\nImmunohistochemistry: 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, 10-20 minutes, followed by cooling at RT for 20 minutes\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\n293 cells or adenovirus infected cells and tissues.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0880-05

Size

500ul

Applications

IF, IHC, IP, WB

Hosts

Mouse

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See A0880-05X.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

1. Harlow, E., et al., Mol. Cell. Biol. 6: 1579-1589 (1986). 2. Harlow, E., et al., J. Virol. 3: 533-546 (1985). 3. Whyte, P., et al., Nature 334: 124-129 (1988). 4. Klein, G., et al., Science 238: 1539-1544 (1987).

Additional Info

Recognizes Adenovirus