Adenovirus, Types 2,5, Early Region (E1A)

The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.\n\nApplications: \nSuitable for use in ELISA, Immunoprecipitation, Immunocytochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 0.05-0.1ug/ml detects the Ad2 and Ad5 E1A gene products in RIPA lysates from HEK293 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0879-05 (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody (HRP) and a chemiluminescence detection system. \nImmunoprecipitation: An independent laboratory has reported A0879-05 immunoprecipitates Ad2 and Ad5 E1A gene products and associated proteins.\nImmunocytochemistry: An independent laboratory has reported thatA0879-05 immunostains Ad2 and Ad5 E1A gene products. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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