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Adenylate Cyclase Type 3 (Adenylate Cyclase Type III, AC-III, Adenylyl Cyclase 3, AC3, Adcy3, Adenylate Cyclase Olfactive Type, ATP Pyrophosphate-lyase 3)

Cat no: 136860

Adenylate Cyclase Type 3 (Adenylate Cyclase Type III, AC-III, Adenylyl Cyclase 3, AC3, Adcy3, Adenylate Cyclase Olfactive Type, ATP Pyrophosphate-lyase 3)

Adenylate cyclases are enzymes which interact with and are activated by the GTP bound alpha subunits of trimeric G-proteins. Activated adenylate cyclases are responsible for the production of the important "second messenger" signalling molecule cyclic-AMP, which is generated from ATP. The type III adenylate cyclase enzyme is localized in the membranes surrounding the cilia in neurons.\n\nAdenylate cyclase type III is a large complex molecule of, in the human, 1145aa with a deduced molecular weight of 129kD. The protein may be variably glycosylated, so that on SDS-PAGE and western blots it runs as a diffuse band of about 160kD in cortex and about 200kD in olfactory epithelium. The molecule has a complex structure, with 12 transmembrane domains and two cyclase domains. Each cyclase domain is immediately C-terminal to 6 transmembrane segments, but only the second, C-terminal cyclase is believed to be catalytically active.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:500-1:1000\nWestern Blot: 1:10,000 (major bands at 160-200kD, depending on the tissue)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

136860

Size

100ul

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Ch/Bird, Fel

Form

Supplied as a liquid.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes rat ADCY3. Species Crossreactivity: feline, chicken, human and mouse.

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